Advanced Metagenomics, Sensors and Photocatalysis for Antimicrobial Resistance Elimination [AMSPARE]
The overall aim of this project is to understand the impact of antibiotic pollution on the environmental microbiology and to design effective measures for monitoring antibiotic levels and to remove them from the waste if they are present. This will be mediated through the engagement with industry and policymakers with the development of policies and regulatory framework based on the data obtained from this project.
The proposal is focused on Common Effluent Treatment Plants (CETPs), as communication with CETP owners and pollution boards has identified a need to for these enterprises. To achieve the overall aim of the project it will be divided into four work packages, each addressing specific objectives that feed into the overall aim.
Work Package 1
Hypothesis: AMR in bacteria is driven by a combination of anthropogenic factors and this influences potential reservoirs for infections and also complex microbial communities in the environment.
Objective 1: to create an AMR map through geochemical and metagenomic analysis.
Objective 2: to determine the influence of anthropogenic factors on microbial communities through their characterisation.
Work Package 2
Hypothesis: novel, optical and electrochemical sensing systems deployed in the CETP system will determine levels of antimicrobials presence in waste and provide an effective monitoring system for environmental release of these compounds.
Objective 1: to develop and validate sensors to a variety of antibiotics that can detect antibiotic in effluent at different concentrations to limit environmental exposure.
Work Package 3
Hypothesis: photocatalytic processing will remove antibiotic residues and kill AMR bacteria already in the effluent and minimising risk of AMR development in the water ways.
Objective 1: to develop and validate photo catalytic technologies for a variety of antibiotics and at different concentrations in the effluent of CETPs.
Work Package 4
Hypothesis: the attainment of a detailed AMR map and effective monitoring systems from this project will engage authorities and industry to support each other in waste water management and create effective regulations and policies.
Objective 1: to consolidate industry and government relationships and create and develop effective strategies to regulate antibiotic waste in the environment.